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cd144 magnetic beads  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd144 magnetic beads
    Cd144 Magnetic Beads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd144+magnetic+beads/CD144+(VE-Cadherin)+MicroBeads%2C+human/pm41668160-72-16-19
    Average 95 stars, based on 70 article reviews
    cd144 magnetic beads - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Purification:

    Article Title: Isogenic monocytes improve the responsiveness of hiPSC cardiac spheroids to cardiac stressors
    Article Snippet: On days 3 and 4, the medium was changed to 2 mL StemPro-34 medium (Gibco 10639011) with 1% Pen/Strep, 1% Glutamax (Gibco 35050061), 200 ng/μL human recombinant vascular endothelial growth factor 165 (Peprotech AF-100-20), and 2 μM forskolin (Abcam ab120058). .. On day 5, iPSC-ECs were purified using CD144 magnetic beads (Miltenyi Biotec 130-097-857) according to the manufacturer’s instructions, resuspended in iPSC-EC medium (Endothelium Growth Medium 2 (Promocell C-22111) with 20% FBS (Gibco 16141079), 1% Pen/Strep and 10 μM SB431542 (Selleckchem S1067)) and transferred to fibronectin (Promocell C-43060) coated T75 flasks. .. The iPSC-EC purity was assessed using flow cytometry (Attune NxT acoustic focusing cytometer (Life Technologies)).

    Magnetic Beads:

    Article Title: Isogenic monocytes improve the responsiveness of hiPSC cardiac spheroids to cardiac stressors
    Article Snippet: On days 3 and 4, the medium was changed to 2 mL StemPro-34 medium (Gibco 10639011) with 1% Pen/Strep, 1% Glutamax (Gibco 35050061), 200 ng/μL human recombinant vascular endothelial growth factor 165 (Peprotech AF-100-20), and 2 μM forskolin (Abcam ab120058). .. On day 5, iPSC-ECs were purified using CD144 magnetic beads (Miltenyi Biotec 130-097-857) according to the manufacturer’s instructions, resuspended in iPSC-EC medium (Endothelium Growth Medium 2 (Promocell C-22111) with 20% FBS (Gibco 16141079), 1% Pen/Strep and 10 μM SB431542 (Selleckchem S1067)) and transferred to fibronectin (Promocell C-43060) coated T75 flasks. .. The iPSC-EC purity was assessed using flow cytometry (Attune NxT acoustic focusing cytometer (Life Technologies)).

    Article Title: Directed Differentiation of Human Induced Pluripotent Stem Cells to Heart Valve Cells
    Article Snippet: .. At the end of differentiation on day 6, the cells were digested with Accutase (Stemcell, 07922, Canada) and sorted with CD144 magnetic beads (Miltenyi Biotec, 130-097-857, Germany), CD144+ cells were further cultured in EGM-2 (LONZA, CC-3202, Switzerland) medium supplemented with 1 μmol/L forskolin, whereas CD144– cells were cultured in DMEM (Gibco, C119955000BT, United States) with 10% fetal bovine serum (Gibco, 10099, United States). ..

    Article Title: 3D Bio‐Printing and Dynamic Maturation of Heart Valve Organoids Made of Human Induced Pluripotent Stem Cell‐Derived Valve Interstitial Cells
    Article Snippet: Heart valve organoids hold promise for modeling human valve biology, yet existing platforms often lack human-relevant cell sources, preserved multicellular architecture, and physiologic mechanical cues, limiting their capacity to recapitulate extracellular matrix (ECM) formation and structural maturation.. Here, a strategy is established that integrates human induced pluripotent stem cell-derived valve interstitial cells (hiPS-VICs), spheroid organization, bio-printable hydrogels, and controlled mechanical conditioning to engineer advanced valve organoids.. 3D bio-printing of hiPS-VIC spheroids is conducted within GelMA/ChSMA constructs, systematically compares spheroid-based bio-printing (SBB) with single-cell bio-printing (SCB), and establishes mechanically-stimulated spheroid-based bio-printing (MSSB) to guide ECM remodeling.

    Article Title: Epigenetic regulation by HDAC11-driven STAT3 activation promotes pro-inflammatory cytokine production and endothelial dysfunction upon bacterial infection in diabetes
    Article Snippet: .. The cell pellet was resuspended with 100 μL of the selection buffer and 40 μL of CD144 magnetic beads (Miltenyi Biotec, Cat. No. 130-097-857) for 15 min and sorted using magnetic-activated cells sorting (MACS). ..

    Article Title: Directed Differentiation of Human Induced Pluripotent Stem Cells to Heart Valve Cells
    Article Snippet: .. At the end of differentiation on day 6, the cells were digested with Accutase (Stemcell, 07922, Canada) and sorted with CD144 magnetic beads (Miltenyi Biotec, 130-097-857, Germany), CD144 + cells were further cultured in EGM-2 (LONZA, CC-3202, Switzerland) medium supplemented with 1 μmol/L forskolin, whereas CD144 – cells were cultured in DMEM (Gibco, C119955000BT, United States) with 10% fetal bovine serum (Gibco, 10099, United States). ..

    Cell Culture:

    Article Title: Directed Differentiation of Human Induced Pluripotent Stem Cells to Heart Valve Cells
    Article Snippet: .. At the end of differentiation on day 6, the cells were digested with Accutase (Stemcell, 07922, Canada) and sorted with CD144 magnetic beads (Miltenyi Biotec, 130-097-857, Germany), CD144+ cells were further cultured in EGM-2 (LONZA, CC-3202, Switzerland) medium supplemented with 1 μmol/L forskolin, whereas CD144– cells were cultured in DMEM (Gibco, C119955000BT, United States) with 10% fetal bovine serum (Gibco, 10099, United States). ..

    Article Title: Directed Differentiation of Human Induced Pluripotent Stem Cells to Heart Valve Cells
    Article Snippet: .. At the end of differentiation on day 6, the cells were digested with Accutase (Stemcell, 07922, Canada) and sorted with CD144 magnetic beads (Miltenyi Biotec, 130-097-857, Germany), CD144 + cells were further cultured in EGM-2 (LONZA, CC-3202, Switzerland) medium supplemented with 1 μmol/L forskolin, whereas CD144 – cells were cultured in DMEM (Gibco, C119955000BT, United States) with 10% fetal bovine serum (Gibco, 10099, United States). ..

    Selection:

    Article Title: Epigenetic regulation by HDAC11-driven STAT3 activation promotes pro-inflammatory cytokine production and endothelial dysfunction upon bacterial infection in diabetes
    Article Snippet: .. The cell pellet was resuspended with 100 μL of the selection buffer and 40 μL of CD144 magnetic beads (Miltenyi Biotec, Cat. No. 130-097-857) for 15 min and sorted using magnetic-activated cells sorting (MACS). ..

    Article Title: Epigenetic regulation by HDAC11-driven STAT3 activation promotes pro-inflammatory cytokine production and endothelial dysfunction upon bacterial infection in diabetes.
    Article Snippet: .. The cell pellet was resuspended with 100 mL of the selection buffer and 40 mL of CD144 magnetic beads (Miltenyi Biotec, Cat. No. 130-097-857) for 15 minutes and sorted using magneticactivated cells sorting (MACS). ..

    Magnetic Cell Separation:

    Article Title: Epigenetic regulation by HDAC11-driven STAT3 activation promotes pro-inflammatory cytokine production and endothelial dysfunction upon bacterial infection in diabetes
    Article Snippet: .. The cell pellet was resuspended with 100 μL of the selection buffer and 40 μL of CD144 magnetic beads (Miltenyi Biotec, Cat. No. 130-097-857) for 15 min and sorted using magnetic-activated cells sorting (MACS). ..

    Article Title: Epigenetic regulation by HDAC11-driven STAT3 activation promotes pro-inflammatory cytokine production and endothelial dysfunction upon bacterial infection in diabetes.
    Article Snippet: .. The cell pellet was resuspended with 100 mL of the selection buffer and 40 mL of CD144 magnetic beads (Miltenyi Biotec, Cat. No. 130-097-857) for 15 minutes and sorted using magneticactivated cells sorting (MACS). ..



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    Miltenyi Biotec cd144 magnetic beads
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    (A) Locations of various PCR amplicons relative to the rs1173771 haplotype region. (B–E) Confirmation of rs1173771 haplotype region deletion in Y4 and 39b cells by PCR (B and C) and Sanger sequencing (D and E). (F) mRNA expression (mean ± SEM) of neighboring genes NPR3, SUB1, and TARS in endothelial cells <t>(CD144</t> + ) differentiated from iPSC Y4 (WT) and rs1173771 LD region-deleted Y4 (ΔLD). n = 8 (WT) and 12 (ΔLD) independently differentiated samples from 3 rounds of differentiation. * p ≤ 0.05, unpaired t test. (G) mRNA expression (mean ± SEM) of NPR3, SUB1, and TARS in endothelial cells (CD144 + ) differentiated from iPSC 39b (WT) and rs1173771 LD region-deleted 39b (ΔLD). n = 6 (WT) and 6 (ΔLD) independent differentiated samples from 3 rounds of differentiation. * p ≤ 0.05, unpaired t test.
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    Miltenyi Biotec beqpcrr cd144 ve cadherin magnetic beads miltenyi biotec
    (A) Locations of various PCR amplicons relative to the rs1173771 haplotype region. (B–E) Confirmation of rs1173771 haplotype region deletion in Y4 and 39b cells by PCR (B and C) and Sanger sequencing (D and E). (F) mRNA expression (mean ± SEM) of neighboring genes NPR3, SUB1, and TARS in endothelial cells <t>(CD144</t> + ) differentiated from iPSC Y4 (WT) and rs1173771 LD region-deleted Y4 (ΔLD). n = 8 (WT) and 12 (ΔLD) independently differentiated samples from 3 rounds of differentiation. * p ≤ 0.05, unpaired t test. (G) mRNA expression (mean ± SEM) of NPR3, SUB1, and TARS in endothelial cells (CD144 + ) differentiated from iPSC 39b (WT) and rs1173771 LD region-deleted 39b (ΔLD). n = 6 (WT) and 6 (ΔLD) independent differentiated samples from 3 rounds of differentiation. * p ≤ 0.05, unpaired t test.
    Beqpcrr Cd144 Ve Cadherin Magnetic Beads Miltenyi Biotec, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (A) Locations of various PCR amplicons relative to the rs1173771 haplotype region. (B–E) Confirmation of rs1173771 haplotype region deletion in Y4 and 39b cells by PCR (B and C) and Sanger sequencing (D and E). (F) mRNA expression (mean ± SEM) of neighboring genes NPR3, SUB1, and TARS in endothelial cells (CD144 + ) differentiated from iPSC Y4 (WT) and rs1173771 LD region-deleted Y4 (ΔLD). n = 8 (WT) and 12 (ΔLD) independently differentiated samples from 3 rounds of differentiation. * p ≤ 0.05, unpaired t test. (G) mRNA expression (mean ± SEM) of NPR3, SUB1, and TARS in endothelial cells (CD144 + ) differentiated from iPSC 39b (WT) and rs1173771 LD region-deleted 39b (ΔLD). n = 6 (WT) and 6 (ΔLD) independent differentiated samples from 3 rounds of differentiation. * p ≤ 0.05, unpaired t test.

    Journal: Cell reports

    Article Title: Physiological role and mechanisms of action for a long noncoding haplotype region

    doi: 10.1016/j.celrep.2025.115805

    Figure Lengend Snippet: (A) Locations of various PCR amplicons relative to the rs1173771 haplotype region. (B–E) Confirmation of rs1173771 haplotype region deletion in Y4 and 39b cells by PCR (B and C) and Sanger sequencing (D and E). (F) mRNA expression (mean ± SEM) of neighboring genes NPR3, SUB1, and TARS in endothelial cells (CD144 + ) differentiated from iPSC Y4 (WT) and rs1173771 LD region-deleted Y4 (ΔLD). n = 8 (WT) and 12 (ΔLD) independently differentiated samples from 3 rounds of differentiation. * p ≤ 0.05, unpaired t test. (G) mRNA expression (mean ± SEM) of NPR3, SUB1, and TARS in endothelial cells (CD144 + ) differentiated from iPSC 39b (WT) and rs1173771 LD region-deleted 39b (ΔLD). n = 6 (WT) and 6 (ΔLD) independent differentiated samples from 3 rounds of differentiation. * p ≤ 0.05, unpaired t test.

    Article Snippet: CD144 (VE-Cadherin) magnetic beads , Miltenyi Biotec , Cat# 130-097-857.

    Techniques: Sequencing, Expressing