Journal: Cell reports
Article Title: Physiological role and mechanisms of action for a long noncoding haplotype region
doi: 10.1016/j.celrep.2025.115805
Figure Lengend Snippet: (A) Locations of various PCR amplicons relative to the rs1173771 haplotype region. (B–E) Confirmation of rs1173771 haplotype region deletion in Y4 and 39b cells by PCR (B and C) and Sanger sequencing (D and E). (F) mRNA expression (mean ± SEM) of neighboring genes NPR3, SUB1, and TARS in endothelial cells (CD144 + ) differentiated from iPSC Y4 (WT) and rs1173771 LD region-deleted Y4 (ΔLD). n = 8 (WT) and 12 (ΔLD) independently differentiated samples from 3 rounds of differentiation. * p ≤ 0.05, unpaired t test. (G) mRNA expression (mean ± SEM) of NPR3, SUB1, and TARS in endothelial cells (CD144 + ) differentiated from iPSC 39b (WT) and rs1173771 LD region-deleted 39b (ΔLD). n = 6 (WT) and 6 (ΔLD) independent differentiated samples from 3 rounds of differentiation. * p ≤ 0.05, unpaired t test.
Article Snippet: CD144 (VE-Cadherin) magnetic beads , Miltenyi Biotec , Cat# 130-097-857.
Techniques: Sequencing, Expressing